Radioimmunoassay of colchicine

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Positive interference in Radioimmunoassay determination of serum total T3

Introduction: Determination of thyrotropin, total and free thyroxine and triiodothyronine are widely used as diagnostic methods for thyroid function evaluation. There have been numerous reports of interferences in thyroid hormone immunoassays. Herein, a prominent positive interference is reported for a radioimmunoassay kit of total triiodothyronine. Materials and Methods: In this study, a total...

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ST056390 Colchicine

REFERENCE: Reference Luduena, R.F., et al., Tubulin structure and biochemistry. Curr. Opin. Cell Biol. 4, 53-57, (1992) abstract Andreu, J.M., et al., Role of the colchicine ring A and its methoxy groups in the binding to tubulin and microtubule inhibition. Biochemistry 37, 8356-8368, (1998) abstract Suzuki, Y, Cell death, phagocytosis, and neurogenesis in mouse olfactory epithelium and vomeron...

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Triiodothyronine radioimmunoassay.

Highly specific antisera to triiodothyronine (T(3)) were prepared by immunization of rabbits with T(3)-bovine serum albumin conjugates. Antisera with T(3) binding capacity of up to 600 ng/ml were obtained. The ability of various thyronine derivatives to inhibit the binding of T(3-) (125)I to anti-T(3) serum was found to vary considerably. l-T(3), d-T(3) and several triiodoanalogues were potent ...

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Rapid radioimmunoassay of triiodothyronine.

The method used in this report is based on the immunological production of a specific antisera to tnlodothyronine (T3) in rabbits. Conducted at 4 #{176}C, the radioimmunoassay requires three to five days to complete. Hoping to speed this assay, we investigated the influence of time, temperature, and pH. Incubation at 37 #{176}C and pH 8.0 for 30 mm did not deleteriously affect either precision,...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1975

ISSN: 0264-6021

DOI: 10.1042/bj1510413